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human pericytes  (PromoCell)


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    PromoCell human pericytes
    Human Pericytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+placenta+pericytes/Human+Pericytes+from+Placenta/pm41650956-926-2-7
    Average 95 stars, based on 97 article reviews
    human pericytes - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence
    Article Snippet: .. Human placenta pericytes (hPL‐PC, C‐12980, PromoCell, Heidelberg, Germany) were cultured in Pericyte Growth Medium 2 (C‐28041, PromoCell) at 37 °C and 5% CO 2 . .. When cells reached around 80–90% confluency, they were split using Accutase (#A6964, Abcam, Cambridge, UK).

    Article Title: Pericyte-expressed Tie2 controls angiogenesis and vessel maturation
    Article Snippet: Human brain vascular pericytes (BP) were purchased from ScienCell (#1200) and cultured in pericyte medium (ScienCell, #1201) supplemented with 2% FCS, 1% of the corresponding pericyte growth supplement and 1% penicillin/streptomycin. .. Human placenta pericytes were purchased from PromoCell (C-12980) and human pancreas (PA), lung (LP) and muscle (MP) pericytes were kindly provided by Dr Bruno Peault (University of Edinburgh, UK) and cultured as described for BP. .. Dermal fibroblasts were purchased from PromoCell (C-12300) and cultured in Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 10% FCS and % penicillin/streptomycin.

    Article Title: STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence
    Article Snippet: .. Human placenta pericytes (hPL-PC, C-12980, PromoCell) were cultured in Pericyte Growth Medium 2 (C-28041, PromoCell) at 37 °C and 5% CO2. .. When cells reached around 80-90% confluency, they were split using Accutase (#A6964, Abcam).

    Article Title: Pericyte-expressed Tie2 controls angiogenesis and vessel maturation.
    Article Snippet: Human brain vascular pericytes (BP) were purchased from ScienCell (#1200) and cultured in pericyte medium (ScienCell, #1201) supplemented with 2% FCS, 1% of the corresponding pericyte growth supplement and 1% penicillin/streptomycin. .. Human placenta pericytes were purchased from PromoCell (C-12980) and human pancreas (PA), lung (LP) and muscle (MP) pericytes were kindly provided by Dr Bruno Peault (University of Edinburgh, UK) and cultured as described for BP. .. Dermal fibroblasts were purchased from PromoCell (C-12300) and cultured in Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 10% FCS and % penicillin/streptomycin.

    Article Title: STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence.
    Article Snippet: .. Human placenta pericytes (hPL-PC, C-12980, PromoCell, Heidelberg, Germany) were cultured in Pericyte Growth Medium 2 (C-28041, PromoCell) at 37 °C and 5% CO2. .. When cells reached around 80–90% confluency, they were split using Accutase (#A6964, Abcam, Cambridge, UK).

    Concentration Assay:

    Article Title: Engineering of functional, perfusable 3D microvascular networks on a chip.
    Article Snippet: Generating perfusable 3D microvessels in vitro is an important goal for tissue engineering, as well as for reliable modelling of blood vessel function.. To date, in vitro blood vessel models have not been able to accurately reproduce the dynamics and responses of endothelial cells to grow perfusable and functional 3D vascular networks.. Here we describe a microfluidic-based platform whereby we model natural cellular programs found during normal development and angiogenesis to form perfusable networks of intact 3D microvessels as well as tumor vasculatures based on the spatially controlled co-culture of endothelial cells with stromal fibroblasts, pericytes or cancer cells.

    Injection:

    Article Title: Engineering of functional, perfusable 3D microvascular networks on a chip.
    Article Snippet: Generating perfusable 3D microvessels in vitro is an important goal for tissue engineering, as well as for reliable modelling of blood vessel function.. To date, in vitro blood vessel models have not been able to accurately reproduce the dynamics and responses of endothelial cells to grow perfusable and functional 3D vascular networks.. Here we describe a microfluidic-based platform whereby we model natural cellular programs found during normal development and angiogenesis to form perfusable networks of intact 3D microvessels as well as tumor vasculatures based on the spatially controlled co-culture of endothelial cells with stromal fibroblasts, pericytes or cancer cells.



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    TGF β -1 signalling via canonical receptor ALK5 in placental <t>pericytes.</t> ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).
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    TGF β -1 signalling via canonical receptor ALK5 in placental <t>pericytes.</t> ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).
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    TGF β -1 signalling via canonical receptor ALK5 in placental <t>pericytes.</t> ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).
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    Image Search Results


    TGF β -1 signalling via canonical receptor ALK5 in placental pericytes. ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: TGF β -1 signalling via canonical receptor ALK5 in placental pericytes. ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Expressing, Clinical Proteomics, Membrane, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

    ELISA analysis of angiogenic factors secreted from placental pericytes. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in; ( a ) VEGF secretion (Kruskal–Wallis analysis); ( b ) MMP-2 secretion (one-way ANOVA). ( c ) ANG-1 secretion (one-way ANOVA). ELISA results normalized to cell number. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: ELISA analysis of angiogenic factors secreted from placental pericytes. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in; ( a ) VEGF secretion (Kruskal–Wallis analysis); ( b ) MMP-2 secretion (one-way ANOVA). ( c ) ANG-1 secretion (one-way ANOVA). ELISA results normalized to cell number. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Enzyme-linked Immunosorbent Assay, Control

    Collagen IV, fibronectin and laminin staining in placental pericytes. Representative ICC/IF images (VEH-treated placental pericytes); ( a ) Collagen IV (red), nuclear stain (blue); data expressed as area and mean fluorescent intensity (MFI); one-way ANOVA, normalized to cell number; ( b ) Fibronectin (red), nuclear stain (blue), data expressed as area and MFI; one-way ANOVA, normalized to cell number; ( c ) Laminin (red), nuclear stain (blue); data expressed as area; Kruskal–Wallis analysis and MFI; one-way ANOVA, normalized to cell number. Scale Bars = 150 μm. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: Collagen IV, fibronectin and laminin staining in placental pericytes. Representative ICC/IF images (VEH-treated placental pericytes); ( a ) Collagen IV (red), nuclear stain (blue); data expressed as area and mean fluorescent intensity (MFI); one-way ANOVA, normalized to cell number; ( b ) Fibronectin (red), nuclear stain (blue), data expressed as area and MFI; one-way ANOVA, normalized to cell number; ( c ) Laminin (red), nuclear stain (blue); data expressed as area; Kruskal–Wallis analysis and MFI; one-way ANOVA, normalized to cell number. Scale Bars = 150 μm. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Staining

    Placental pericyte secretion of inflammatory factors. Analysis of inflammatory secretion (ELISA). Treatment groups: VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( a ) IL-6 secretion; one-way ANOVA; ( b ) MCP-1 secretion; one-way ANOVA; ( c ) sVCAM-1 secretion; one-way ANOVA; ( d ) CX3CL1 secretion; one-way ANOVA. ( e ) IL-8 secretion; Kruskal–Wallis analysis. Data (n = 3); stars indicate statistical significance between treatment groups ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: Placental pericyte secretion of inflammatory factors. Analysis of inflammatory secretion (ELISA). Treatment groups: VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( a ) IL-6 secretion; one-way ANOVA; ( b ) MCP-1 secretion; one-way ANOVA; ( c ) sVCAM-1 secretion; one-way ANOVA; ( d ) CX3CL1 secretion; one-way ANOVA. ( e ) IL-8 secretion; Kruskal–Wallis analysis. Data (n = 3); stars indicate statistical significance between treatment groups ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Enzyme-linked Immunosorbent Assay, Control

    Alternate TGF β -1 signalling pathway in placental pericytes: Type I receptor ACVRL1 (ALK1, 94 bp) expression in placental pericytes, as assessed by RT-PCR; original image shown.

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: Alternate TGF β -1 signalling pathway in placental pericytes: Type I receptor ACVRL1 (ALK1, 94 bp) expression in placental pericytes, as assessed by RT-PCR; original image shown.

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Phagocytosis capacity of placental pericytes. Treatment groups: VEH control and TGF β -1 (10 ng/mL) with and without FluoSphere incubation; ( a ) Representative flow cytometry histograms to identify the percentage of the population with and without phagocytosed FluoSpheres based on fluorescence; ( b ) Analysis of phagocytosis (Mann–Whitney). Data (n = 3) expressed as mean ± SEM. Stars indicate statistical significance, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; c Fluorescent and phase contrast images of pericyte phagocytosis, nuclei (blue), FluoSphere (green). Yellow arrows identify pericytes with phagocytosed beads, and red arrows identify beads that have not been phagocytosed. Scale bar = 150 μm.

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: Phagocytosis capacity of placental pericytes. Treatment groups: VEH control and TGF β -1 (10 ng/mL) with and without FluoSphere incubation; ( a ) Representative flow cytometry histograms to identify the percentage of the population with and without phagocytosed FluoSpheres based on fluorescence; ( b ) Analysis of phagocytosis (Mann–Whitney). Data (n = 3) expressed as mean ± SEM. Stars indicate statistical significance, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; c Fluorescent and phase contrast images of pericyte phagocytosis, nuclei (blue), FluoSphere (green). Yellow arrows identify pericytes with phagocytosed beads, and red arrows identify beads that have not been phagocytosed. Scale bar = 150 μm.

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Control, Incubation, Flow Cytometry, Fluorescence, MANN-WHITNEY